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Promega fugene 6 transfection reagent kit
Fugene 6 Transfection Reagent Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fugene+6+transfection+reagent+kit/fugene+hd/pmc11406263__pnas__2408262121__sapp-23-5-10
Average 90 stars, based on 1 article reviews
fugene 6 transfection reagent kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Transfection:

Article Title: A familial missense variant in the Alzheimer’s disease gene SORL1 impairs its maturation and endosomal sorting
Article Snippet: .. Approximately 5 × 10 5 HEK293 and N2a cells were seeded on 6-well plates and transiently transfected with expression constructs for SORL1 -WT or SORL1 -R953C, using Fugene 6 Transfection Reagent kit (Promega) according to the manufacturer’s instructions. ..

Article Title: A familial missense variant in the Alzheimer's disease gene SORL1 impairs its maturation and endosomal sorting.
Article Snippet: Results were analyzed using FlowJoTM v10.8.1 Software (BD Life Sciences). .. Approximately 5 × 104 HEK293 cells were seeded on poly-L-lysine-coated glass coverslips and transfected with expression constructs for SORL1-WT or SORL1-R953C using Fugene 6 Transfection Reagent kit (Promega). ..

Article Title: The SORL1 p.Y1816C variant causes impaired endosomal dimerization and autosomal dominant Alzheimer's disease.
Article Snippet: Increasing evidence points to SORL1 as the fourth causal Alzheimer’s disease (AD) gene.. Missense variants in SORL1 have continuously been found to be associated with AD.. Here, we describe a SORL1 variant, p.Y1816C, in three unrelated families, each with a history of AD.

Article Title: Functional characterization of SORL1 variants in cell-based assays to investigate variant pathogenicity
Article Snippet: .. Approximately 5 × 10 5 HEK293 or N2a cells were seeded on six-well plates and transfected with expression constructs for SORLA-WT or SORLA-D1105H, using the Fugene 6 Transfection Reagent kit (Promega) according to the manufacturer's instructions. ..

Article Title: Supporting Information for The SORL1 p.Y1816C variant causes impaired endosomal dimerization and autosomal dominant Alzheimer’s disease
Article Snippet: HEK293 cells (ATCC) were cultured in Dulbecco’s Modified Eagles Medium (Gibco) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin in a humidified 5% CO2 incubator at 37°C. .. Transient transfections were performed using Fugene 6 Transfection Reagent kit (Promega) with a Fugene:DNA ratio of 3:1. ..

Article Title: Functional characterization of SORL1 variants in cell-based assays to investigate variant pathogenicity
Article Snippet: Results were analysed using FlowJo v10.8.1 software (BD Life Sciences) and data were plotted in GraphPad Prism 9.5.0. .. Approximately 5 × 10 4 HEK293 cells were seeded on poly-L-lysine-coated glass coverslips and were then transfected with plasmid constructs expressing SORLA-WT or SORLA-D1105H using the Fugene 6 Transfection Reagent kit (Promega). ..

Article Title: A familial missense variant in the Alzheimer's disease gene SORL1 impairs its maturation and endosomal sorting.
Article Snippet: .. Approximately 5 × 105 HEK293 and N2a cells were seeded on 6-well plates and transiently transfected with expression constructs for SORL1-WT or SORL1-R953C, using Fugene 6 Transfection Reagent kit (Promega) according to the manufacturer’s instructions. ..

Article Title: Pharmacological rescue of mutant p53 triggers spontaneous tumor regression via immune responses.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Waf1/Cip1/CDKN1A p21 Antibody (187) Santa Cruz Biotechnology Cat# sc-817; RRID: AB_628072 Anti-p53 antibody Abcam Cat# ab26; RRID: AB_303198 beta Actin Antibody Santa Cruz Biotechnology Cat# sc-47778 HRP; RRID: AB_2714189 Monoclonal Anti-MDM2 antibody Sigma-Aldrich Cat# M4308; RRID: AB_260523 ISG15 antibody Proteintech Cat# 15981-1-AP; RRID: AB_2126302 IFIT1 antibody Proteintech Cat# 23247-1-AP; RRID: AB_2811269 GAPDH Mouse mAb ABclonal Cat# AC002; RRID: AB_2736879 Ki67 Rabbit mAb Beyotime Cat# AF1738; RRID: AB_3094450 goat anti-rabbit immunoglobulin Servicebio Cat# GB23303; RRID: AB_2811189 CD4+ depletion antibody BioLegend Cat# 100457; RRID: AB_2810318 CD8+ depletion antibody BioLegend Cat# 100763; RRID: AB_2810323 IFN-g depletion antibody BioLegend Cat# 505847; RRID: AB_2616675 CD45.1-FITC antibody BioLegend Cat# 110705; RRID: AB_313494 CD3-PE/Cyanine7 antibody BioLegend Cat# 100219; RRID: AB_1732068 CD19-Brilliant Violet 510 antibody BioLegend Cat# 115545; RRID: AB_2562136 CD4-FITC antibody BioLegend Cat# 100405; RRID: AB_312690 CD4-APC/Cyanine7 antibody BioLegend Cat# 100413; RRID: AB_312698 CD8a-PE antibody BioLegend Cat# 100707; RRID: AB_312746 CD11B-PE/Cyanine7 antibody BioLegend Cat# 101215; RRID: AB_312798 CD11C-PerCP antibody BioLegend Cat# 117325; RRID: AB_893236 F4/80-Brilliant Violet 510 antibody BioLegend Cat# 123135; RRID: AB_2562622 NK1.1-APC antibody BioLegend Cat# 156505; RRID: AB_2876525 Bacterial and virus strains Escherichia coli DH5a TIANGEN Cat# CB101-02 Chemicals, peptides, and recombinant proteins Arsenic trioxide (ATO) Sigma Cat# 202673 100 3 L-Glutamine Sangon Cat# E607004 DMEM, High Glucose, Pyruvate Thermo Fisher Cat# 11995073 BASIC RPMI 1640 Medium, HEPES Thermo Fisher Cat# 22400105 Opti-MEM I Reduced Serum Medium, GlutaMAXTM Thermo Fisher Cat# 51985-034 Penicillin-Streptomycin Thermo Fisher Cat# 15140-122 Fetal bovine serum Moregate Cat# FBSF-500 Critical commercial assays Dual Luciferase Reporter Assay Kit Vazyme Cat# DL101-01 FuGENE 6 transfection reagent kit Promega Cat# E2691 RIPA buffer Beyotime Cat# P0013B TRIzol reagent Thermo Fisher Cat# 15596018CN HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) Vazyme Cat# R223-01 ChamQTM SYBR qPCR Master Mix Vazyme Cat# Q331-02/03 BSA Yeasen Cat# 36101ES80 HilyMax transfection reagent kit Dojindo Cat# H357 (Continued on next page) Cell Reports Medicine 6, 101976, March 18, 2025 e1 ..

Expressing:

Article Title: A familial missense variant in the Alzheimer’s disease gene SORL1 impairs its maturation and endosomal sorting
Article Snippet: .. Approximately 5 × 10 5 HEK293 and N2a cells were seeded on 6-well plates and transiently transfected with expression constructs for SORL1 -WT or SORL1 -R953C, using Fugene 6 Transfection Reagent kit (Promega) according to the manufacturer’s instructions. ..

Article Title: A familial missense variant in the Alzheimer's disease gene SORL1 impairs its maturation and endosomal sorting.
Article Snippet: Results were analyzed using FlowJoTM v10.8.1 Software (BD Life Sciences). .. Approximately 5 × 104 HEK293 cells were seeded on poly-L-lysine-coated glass coverslips and transfected with expression constructs for SORL1-WT or SORL1-R953C using Fugene 6 Transfection Reagent kit (Promega). ..

Article Title: Functional characterization of SORL1 variants in cell-based assays to investigate variant pathogenicity
Article Snippet: .. Approximately 5 × 10 5 HEK293 or N2a cells were seeded on six-well plates and transfected with expression constructs for SORLA-WT or SORLA-D1105H, using the Fugene 6 Transfection Reagent kit (Promega) according to the manufacturer's instructions. ..

Article Title: Functional characterization of SORL1 variants in cell-based assays to investigate variant pathogenicity
Article Snippet: Results were analysed using FlowJo v10.8.1 software (BD Life Sciences) and data were plotted in GraphPad Prism 9.5.0. .. Approximately 5 × 10 4 HEK293 cells were seeded on poly-L-lysine-coated glass coverslips and were then transfected with plasmid constructs expressing SORLA-WT or SORLA-D1105H using the Fugene 6 Transfection Reagent kit (Promega). ..

Article Title: A familial missense variant in the Alzheimer's disease gene SORL1 impairs its maturation and endosomal sorting.
Article Snippet: .. Approximately 5 × 105 HEK293 and N2a cells were seeded on 6-well plates and transiently transfected with expression constructs for SORL1-WT or SORL1-R953C, using Fugene 6 Transfection Reagent kit (Promega) according to the manufacturer’s instructions. ..

Construct:

Article Title: A familial missense variant in the Alzheimer’s disease gene SORL1 impairs its maturation and endosomal sorting
Article Snippet: .. Approximately 5 × 10 5 HEK293 and N2a cells were seeded on 6-well plates and transiently transfected with expression constructs for SORL1 -WT or SORL1 -R953C, using Fugene 6 Transfection Reagent kit (Promega) according to the manufacturer’s instructions. ..

Article Title: A familial missense variant in the Alzheimer's disease gene SORL1 impairs its maturation and endosomal sorting.
Article Snippet: Results were analyzed using FlowJoTM v10.8.1 Software (BD Life Sciences). .. Approximately 5 × 104 HEK293 cells were seeded on poly-L-lysine-coated glass coverslips and transfected with expression constructs for SORL1-WT or SORL1-R953C using Fugene 6 Transfection Reagent kit (Promega). ..

Article Title: Functional characterization of SORL1 variants in cell-based assays to investigate variant pathogenicity
Article Snippet: .. Approximately 5 × 10 5 HEK293 or N2a cells were seeded on six-well plates and transfected with expression constructs for SORLA-WT or SORLA-D1105H, using the Fugene 6 Transfection Reagent kit (Promega) according to the manufacturer's instructions. ..

Article Title: Functional characterization of SORL1 variants in cell-based assays to investigate variant pathogenicity
Article Snippet: Results were analysed using FlowJo v10.8.1 software (BD Life Sciences) and data were plotted in GraphPad Prism 9.5.0. .. Approximately 5 × 10 4 HEK293 cells were seeded on poly-L-lysine-coated glass coverslips and were then transfected with plasmid constructs expressing SORLA-WT or SORLA-D1105H using the Fugene 6 Transfection Reagent kit (Promega). ..

Article Title: A familial missense variant in the Alzheimer's disease gene SORL1 impairs its maturation and endosomal sorting.
Article Snippet: .. Approximately 5 × 105 HEK293 and N2a cells were seeded on 6-well plates and transiently transfected with expression constructs for SORL1-WT or SORL1-R953C, using Fugene 6 Transfection Reagent kit (Promega) according to the manufacturer’s instructions. ..

Plasmid Preparation:

Article Title: Functional characterization of SORL1 variants in cell-based assays to investigate variant pathogenicity
Article Snippet: Results were analysed using FlowJo v10.8.1 software (BD Life Sciences) and data were plotted in GraphPad Prism 9.5.0. .. Approximately 5 × 10 4 HEK293 cells were seeded on poly-L-lysine-coated glass coverslips and were then transfected with plasmid constructs expressing SORLA-WT or SORLA-D1105H using the Fugene 6 Transfection Reagent kit (Promega). ..

Virus:

Article Title: Pharmacological rescue of mutant p53 triggers spontaneous tumor regression via immune responses.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Waf1/Cip1/CDKN1A p21 Antibody (187) Santa Cruz Biotechnology Cat# sc-817; RRID: AB_628072 Anti-p53 antibody Abcam Cat# ab26; RRID: AB_303198 beta Actin Antibody Santa Cruz Biotechnology Cat# sc-47778 HRP; RRID: AB_2714189 Monoclonal Anti-MDM2 antibody Sigma-Aldrich Cat# M4308; RRID: AB_260523 ISG15 antibody Proteintech Cat# 15981-1-AP; RRID: AB_2126302 IFIT1 antibody Proteintech Cat# 23247-1-AP; RRID: AB_2811269 GAPDH Mouse mAb ABclonal Cat# AC002; RRID: AB_2736879 Ki67 Rabbit mAb Beyotime Cat# AF1738; RRID: AB_3094450 goat anti-rabbit immunoglobulin Servicebio Cat# GB23303; RRID: AB_2811189 CD4+ depletion antibody BioLegend Cat# 100457; RRID: AB_2810318 CD8+ depletion antibody BioLegend Cat# 100763; RRID: AB_2810323 IFN-g depletion antibody BioLegend Cat# 505847; RRID: AB_2616675 CD45.1-FITC antibody BioLegend Cat# 110705; RRID: AB_313494 CD3-PE/Cyanine7 antibody BioLegend Cat# 100219; RRID: AB_1732068 CD19-Brilliant Violet 510 antibody BioLegend Cat# 115545; RRID: AB_2562136 CD4-FITC antibody BioLegend Cat# 100405; RRID: AB_312690 CD4-APC/Cyanine7 antibody BioLegend Cat# 100413; RRID: AB_312698 CD8a-PE antibody BioLegend Cat# 100707; RRID: AB_312746 CD11B-PE/Cyanine7 antibody BioLegend Cat# 101215; RRID: AB_312798 CD11C-PerCP antibody BioLegend Cat# 117325; RRID: AB_893236 F4/80-Brilliant Violet 510 antibody BioLegend Cat# 123135; RRID: AB_2562622 NK1.1-APC antibody BioLegend Cat# 156505; RRID: AB_2876525 Bacterial and virus strains Escherichia coli DH5a TIANGEN Cat# CB101-02 Chemicals, peptides, and recombinant proteins Arsenic trioxide (ATO) Sigma Cat# 202673 100 3 L-Glutamine Sangon Cat# E607004 DMEM, High Glucose, Pyruvate Thermo Fisher Cat# 11995073 BASIC RPMI 1640 Medium, HEPES Thermo Fisher Cat# 22400105 Opti-MEM I Reduced Serum Medium, GlutaMAXTM Thermo Fisher Cat# 51985-034 Penicillin-Streptomycin Thermo Fisher Cat# 15140-122 Fetal bovine serum Moregate Cat# FBSF-500 Critical commercial assays Dual Luciferase Reporter Assay Kit Vazyme Cat# DL101-01 FuGENE 6 transfection reagent kit Promega Cat# E2691 RIPA buffer Beyotime Cat# P0013B TRIzol reagent Thermo Fisher Cat# 15596018CN HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) Vazyme Cat# R223-01 ChamQTM SYBR qPCR Master Mix Vazyme Cat# Q331-02/03 BSA Yeasen Cat# 36101ES80 HilyMax transfection reagent kit Dojindo Cat# H357 (Continued on next page) Cell Reports Medicine 6, 101976, March 18, 2025 e1 ..

Recombinant:

Article Title: Pharmacological rescue of mutant p53 triggers spontaneous tumor regression via immune responses.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Waf1/Cip1/CDKN1A p21 Antibody (187) Santa Cruz Biotechnology Cat# sc-817; RRID: AB_628072 Anti-p53 antibody Abcam Cat# ab26; RRID: AB_303198 beta Actin Antibody Santa Cruz Biotechnology Cat# sc-47778 HRP; RRID: AB_2714189 Monoclonal Anti-MDM2 antibody Sigma-Aldrich Cat# M4308; RRID: AB_260523 ISG15 antibody Proteintech Cat# 15981-1-AP; RRID: AB_2126302 IFIT1 antibody Proteintech Cat# 23247-1-AP; RRID: AB_2811269 GAPDH Mouse mAb ABclonal Cat# AC002; RRID: AB_2736879 Ki67 Rabbit mAb Beyotime Cat# AF1738; RRID: AB_3094450 goat anti-rabbit immunoglobulin Servicebio Cat# GB23303; RRID: AB_2811189 CD4+ depletion antibody BioLegend Cat# 100457; RRID: AB_2810318 CD8+ depletion antibody BioLegend Cat# 100763; RRID: AB_2810323 IFN-g depletion antibody BioLegend Cat# 505847; RRID: AB_2616675 CD45.1-FITC antibody BioLegend Cat# 110705; RRID: AB_313494 CD3-PE/Cyanine7 antibody BioLegend Cat# 100219; RRID: AB_1732068 CD19-Brilliant Violet 510 antibody BioLegend Cat# 115545; RRID: AB_2562136 CD4-FITC antibody BioLegend Cat# 100405; RRID: AB_312690 CD4-APC/Cyanine7 antibody BioLegend Cat# 100413; RRID: AB_312698 CD8a-PE antibody BioLegend Cat# 100707; RRID: AB_312746 CD11B-PE/Cyanine7 antibody BioLegend Cat# 101215; RRID: AB_312798 CD11C-PerCP antibody BioLegend Cat# 117325; RRID: AB_893236 F4/80-Brilliant Violet 510 antibody BioLegend Cat# 123135; RRID: AB_2562622 NK1.1-APC antibody BioLegend Cat# 156505; RRID: AB_2876525 Bacterial and virus strains Escherichia coli DH5a TIANGEN Cat# CB101-02 Chemicals, peptides, and recombinant proteins Arsenic trioxide (ATO) Sigma Cat# 202673 100 3 L-Glutamine Sangon Cat# E607004 DMEM, High Glucose, Pyruvate Thermo Fisher Cat# 11995073 BASIC RPMI 1640 Medium, HEPES Thermo Fisher Cat# 22400105 Opti-MEM I Reduced Serum Medium, GlutaMAXTM Thermo Fisher Cat# 51985-034 Penicillin-Streptomycin Thermo Fisher Cat# 15140-122 Fetal bovine serum Moregate Cat# FBSF-500 Critical commercial assays Dual Luciferase Reporter Assay Kit Vazyme Cat# DL101-01 FuGENE 6 transfection reagent kit Promega Cat# E2691 RIPA buffer Beyotime Cat# P0013B TRIzol reagent Thermo Fisher Cat# 15596018CN HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) Vazyme Cat# R223-01 ChamQTM SYBR qPCR Master Mix Vazyme Cat# Q331-02/03 BSA Yeasen Cat# 36101ES80 HilyMax transfection reagent kit Dojindo Cat# H357 (Continued on next page) Cell Reports Medicine 6, 101976, March 18, 2025 e1 ..

Luciferase:

Article Title: Pharmacological rescue of mutant p53 triggers spontaneous tumor regression via immune responses.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Waf1/Cip1/CDKN1A p21 Antibody (187) Santa Cruz Biotechnology Cat# sc-817; RRID: AB_628072 Anti-p53 antibody Abcam Cat# ab26; RRID: AB_303198 beta Actin Antibody Santa Cruz Biotechnology Cat# sc-47778 HRP; RRID: AB_2714189 Monoclonal Anti-MDM2 antibody Sigma-Aldrich Cat# M4308; RRID: AB_260523 ISG15 antibody Proteintech Cat# 15981-1-AP; RRID: AB_2126302 IFIT1 antibody Proteintech Cat# 23247-1-AP; RRID: AB_2811269 GAPDH Mouse mAb ABclonal Cat# AC002; RRID: AB_2736879 Ki67 Rabbit mAb Beyotime Cat# AF1738; RRID: AB_3094450 goat anti-rabbit immunoglobulin Servicebio Cat# GB23303; RRID: AB_2811189 CD4+ depletion antibody BioLegend Cat# 100457; RRID: AB_2810318 CD8+ depletion antibody BioLegend Cat# 100763; RRID: AB_2810323 IFN-g depletion antibody BioLegend Cat# 505847; RRID: AB_2616675 CD45.1-FITC antibody BioLegend Cat# 110705; RRID: AB_313494 CD3-PE/Cyanine7 antibody BioLegend Cat# 100219; RRID: AB_1732068 CD19-Brilliant Violet 510 antibody BioLegend Cat# 115545; RRID: AB_2562136 CD4-FITC antibody BioLegend Cat# 100405; RRID: AB_312690 CD4-APC/Cyanine7 antibody BioLegend Cat# 100413; RRID: AB_312698 CD8a-PE antibody BioLegend Cat# 100707; RRID: AB_312746 CD11B-PE/Cyanine7 antibody BioLegend Cat# 101215; RRID: AB_312798 CD11C-PerCP antibody BioLegend Cat# 117325; RRID: AB_893236 F4/80-Brilliant Violet 510 antibody BioLegend Cat# 123135; RRID: AB_2562622 NK1.1-APC antibody BioLegend Cat# 156505; RRID: AB_2876525 Bacterial and virus strains Escherichia coli DH5a TIANGEN Cat# CB101-02 Chemicals, peptides, and recombinant proteins Arsenic trioxide (ATO) Sigma Cat# 202673 100 3 L-Glutamine Sangon Cat# E607004 DMEM, High Glucose, Pyruvate Thermo Fisher Cat# 11995073 BASIC RPMI 1640 Medium, HEPES Thermo Fisher Cat# 22400105 Opti-MEM I Reduced Serum Medium, GlutaMAXTM Thermo Fisher Cat# 51985-034 Penicillin-Streptomycin Thermo Fisher Cat# 15140-122 Fetal bovine serum Moregate Cat# FBSF-500 Critical commercial assays Dual Luciferase Reporter Assay Kit Vazyme Cat# DL101-01 FuGENE 6 transfection reagent kit Promega Cat# E2691 RIPA buffer Beyotime Cat# P0013B TRIzol reagent Thermo Fisher Cat# 15596018CN HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) Vazyme Cat# R223-01 ChamQTM SYBR qPCR Master Mix Vazyme Cat# Q331-02/03 BSA Yeasen Cat# 36101ES80 HilyMax transfection reagent kit Dojindo Cat# H357 (Continued on next page) Cell Reports Medicine 6, 101976, March 18, 2025 e1 ..

Reporter Assay:

Article Title: Pharmacological rescue of mutant p53 triggers spontaneous tumor regression via immune responses.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Waf1/Cip1/CDKN1A p21 Antibody (187) Santa Cruz Biotechnology Cat# sc-817; RRID: AB_628072 Anti-p53 antibody Abcam Cat# ab26; RRID: AB_303198 beta Actin Antibody Santa Cruz Biotechnology Cat# sc-47778 HRP; RRID: AB_2714189 Monoclonal Anti-MDM2 antibody Sigma-Aldrich Cat# M4308; RRID: AB_260523 ISG15 antibody Proteintech Cat# 15981-1-AP; RRID: AB_2126302 IFIT1 antibody Proteintech Cat# 23247-1-AP; RRID: AB_2811269 GAPDH Mouse mAb ABclonal Cat# AC002; RRID: AB_2736879 Ki67 Rabbit mAb Beyotime Cat# AF1738; RRID: AB_3094450 goat anti-rabbit immunoglobulin Servicebio Cat# GB23303; RRID: AB_2811189 CD4+ depletion antibody BioLegend Cat# 100457; RRID: AB_2810318 CD8+ depletion antibody BioLegend Cat# 100763; RRID: AB_2810323 IFN-g depletion antibody BioLegend Cat# 505847; RRID: AB_2616675 CD45.1-FITC antibody BioLegend Cat# 110705; RRID: AB_313494 CD3-PE/Cyanine7 antibody BioLegend Cat# 100219; RRID: AB_1732068 CD19-Brilliant Violet 510 antibody BioLegend Cat# 115545; RRID: AB_2562136 CD4-FITC antibody BioLegend Cat# 100405; RRID: AB_312690 CD4-APC/Cyanine7 antibody BioLegend Cat# 100413; RRID: AB_312698 CD8a-PE antibody BioLegend Cat# 100707; RRID: AB_312746 CD11B-PE/Cyanine7 antibody BioLegend Cat# 101215; RRID: AB_312798 CD11C-PerCP antibody BioLegend Cat# 117325; RRID: AB_893236 F4/80-Brilliant Violet 510 antibody BioLegend Cat# 123135; RRID: AB_2562622 NK1.1-APC antibody BioLegend Cat# 156505; RRID: AB_2876525 Bacterial and virus strains Escherichia coli DH5a TIANGEN Cat# CB101-02 Chemicals, peptides, and recombinant proteins Arsenic trioxide (ATO) Sigma Cat# 202673 100 3 L-Glutamine Sangon Cat# E607004 DMEM, High Glucose, Pyruvate Thermo Fisher Cat# 11995073 BASIC RPMI 1640 Medium, HEPES Thermo Fisher Cat# 22400105 Opti-MEM I Reduced Serum Medium, GlutaMAXTM Thermo Fisher Cat# 51985-034 Penicillin-Streptomycin Thermo Fisher Cat# 15140-122 Fetal bovine serum Moregate Cat# FBSF-500 Critical commercial assays Dual Luciferase Reporter Assay Kit Vazyme Cat# DL101-01 FuGENE 6 transfection reagent kit Promega Cat# E2691 RIPA buffer Beyotime Cat# P0013B TRIzol reagent Thermo Fisher Cat# 15596018CN HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) Vazyme Cat# R223-01 ChamQTM SYBR qPCR Master Mix Vazyme Cat# Q331-02/03 BSA Yeasen Cat# 36101ES80 HilyMax transfection reagent kit Dojindo Cat# H357 (Continued on next page) Cell Reports Medicine 6, 101976, March 18, 2025 e1 ..

Real-time Polymerase Chain Reaction:

Article Title: Pharmacological rescue of mutant p53 triggers spontaneous tumor regression via immune responses.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Waf1/Cip1/CDKN1A p21 Antibody (187) Santa Cruz Biotechnology Cat# sc-817; RRID: AB_628072 Anti-p53 antibody Abcam Cat# ab26; RRID: AB_303198 beta Actin Antibody Santa Cruz Biotechnology Cat# sc-47778 HRP; RRID: AB_2714189 Monoclonal Anti-MDM2 antibody Sigma-Aldrich Cat# M4308; RRID: AB_260523 ISG15 antibody Proteintech Cat# 15981-1-AP; RRID: AB_2126302 IFIT1 antibody Proteintech Cat# 23247-1-AP; RRID: AB_2811269 GAPDH Mouse mAb ABclonal Cat# AC002; RRID: AB_2736879 Ki67 Rabbit mAb Beyotime Cat# AF1738; RRID: AB_3094450 goat anti-rabbit immunoglobulin Servicebio Cat# GB23303; RRID: AB_2811189 CD4+ depletion antibody BioLegend Cat# 100457; RRID: AB_2810318 CD8+ depletion antibody BioLegend Cat# 100763; RRID: AB_2810323 IFN-g depletion antibody BioLegend Cat# 505847; RRID: AB_2616675 CD45.1-FITC antibody BioLegend Cat# 110705; RRID: AB_313494 CD3-PE/Cyanine7 antibody BioLegend Cat# 100219; RRID: AB_1732068 CD19-Brilliant Violet 510 antibody BioLegend Cat# 115545; RRID: AB_2562136 CD4-FITC antibody BioLegend Cat# 100405; RRID: AB_312690 CD4-APC/Cyanine7 antibody BioLegend Cat# 100413; RRID: AB_312698 CD8a-PE antibody BioLegend Cat# 100707; RRID: AB_312746 CD11B-PE/Cyanine7 antibody BioLegend Cat# 101215; RRID: AB_312798 CD11C-PerCP antibody BioLegend Cat# 117325; RRID: AB_893236 F4/80-Brilliant Violet 510 antibody BioLegend Cat# 123135; RRID: AB_2562622 NK1.1-APC antibody BioLegend Cat# 156505; RRID: AB_2876525 Bacterial and virus strains Escherichia coli DH5a TIANGEN Cat# CB101-02 Chemicals, peptides, and recombinant proteins Arsenic trioxide (ATO) Sigma Cat# 202673 100 3 L-Glutamine Sangon Cat# E607004 DMEM, High Glucose, Pyruvate Thermo Fisher Cat# 11995073 BASIC RPMI 1640 Medium, HEPES Thermo Fisher Cat# 22400105 Opti-MEM I Reduced Serum Medium, GlutaMAXTM Thermo Fisher Cat# 51985-034 Penicillin-Streptomycin Thermo Fisher Cat# 15140-122 Fetal bovine serum Moregate Cat# FBSF-500 Critical commercial assays Dual Luciferase Reporter Assay Kit Vazyme Cat# DL101-01 FuGENE 6 transfection reagent kit Promega Cat# E2691 RIPA buffer Beyotime Cat# P0013B TRIzol reagent Thermo Fisher Cat# 15596018CN HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) Vazyme Cat# R223-01 ChamQTM SYBR qPCR Master Mix Vazyme Cat# Q331-02/03 BSA Yeasen Cat# 36101ES80 HilyMax transfection reagent kit Dojindo Cat# H357 (Continued on next page) Cell Reports Medicine 6, 101976, March 18, 2025 e1 ..



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Image Search Results


Journal: Cell Reports Medicine

Article Title: Pharmacological rescue of mutant p53 triggers spontaneous tumor regression via immune responses

doi: 10.1016/j.xcrm.2025.101976

Figure Lengend Snippet:

Article Snippet: FuGENE®6 transfection reagent kit , Promega , Cat# E2691.

Techniques: Virus, Recombinant, Luciferase, Reporter Assay, Transfection, Real-time Polymerase Chain Reaction, Expressing, Software